oncoReveal®
Essential LBx Panel
The oncoReveal® Essential LBx panel is a robust NGS assay that interrogates 197 targets across 34 genes of interest from multiple solid tumor cancer types. This is a focused panel that can detect four types of variants from cell-free DNA (cfDNA) that has been extracted from plasma: single nucleotide variants (SNVs), small insertion/deletion (indel) variants, copy number amplification (CNA), and microsatellite instability (MSI). The panel uses proprietary Stem-Loop Inhibition-Mediated amplification (SLIMamp®) technology, a tiled amplicon-based library prep chemistry, designed by Pillar’s AI-empowered VersaTile™ Primer Design tool, for efficient single-tube target enrichment.
oncoReveal® Essential LBx Panel (34 genes)
| AKT1 | CTNNB1 | FGFR2 | KIT | PPP2R1A | SF3B1 |
| ALK | EGFR | FGFR3 | KRAS | PTCH1 | SMAD4 |
| AR | ▲ ERBB2 | GNA11 | ▲ MET | PTEN | TERT (promoter) |
| ATM | ERBB3 | GNAQ | NRAS | RAC1 | TP53 |
| BRAF | ESR1 | GNAS | PDGFRA | RET | |
| CDK4 | ▲ FGFR1 | HRAS | PIK3CA | RNF43 |
| AKT1 | FGFR2 | PPP2R1A |
| ALK | FGFR3 | PTCH1 |
| AR | GNA11 | PTEN |
| ATM | GNAQ | RAC1 |
| BRAF | GNAS | RET |
| CDK4 | HRAS | RNF43 |
| CTNNB1 | KIT | SF3B1 |
| EGFR | KRAS | SMAD4 |
| ▲ ERBB2 | ▲ MET | TERT (promoter) |
| ERBB3 | NRAS | TP53 |
| ESR1 | PDGFRA | |
| ▲ FGFR1 | PIK3CA |
Copy Number Amplifications (CNAs) can also be detected in genes indicated by ▲
Genes marked in green indicate full CDS coverage
Simple NGS Library Prep Workflow
Maintain control of samples and results with single-tube, tiled amplification that can be performed in-house by any NGS labSensitive and Robust Chemistry
Achieve variant detection as low as 1% VAF* even with limited DNA input or poor sample qualityReduced Fully-Loaded Lab Costs
Improve lab efficiency and reduce “no calls”, repeat testing, and difficult interpretation decisions*VAF, variant allele frequency
For Research Use Only. Not for use in diagnostic procedures..
Panel Specifications*
| Enrichment chemistry | Multiplex PCR using tiled amplicons |
| Number of pools | 1 pool |
| Number of genes/amplicons | 34/201 |
| Number of targets | Hotspots in 33 genes; full CDS coverage of TP53; 24 MSI sites; CNAs in 3 genes |
| Variant types | SNVs, indels, CNAs, and MSI |
| Average amplicon size | 90bp |
| Recommended DNA input range | 10ng to 30ng |
| Sample types | cfDNA from plasma |
| Mapping rate | ≥90% |
| % on-target aligned reads | ≥85% |
| Coverage uniformity | |
| (% targets with >0.2X mean coverage) | ≥90% |
| Recommended Reads Per Sample | ~10 million paired-end reads |
| Total assay time (from DNA to sequencer) | <8 hours |
Ordering Information
Select the panel and one of the index kit options listed below.
| Panel | Part number |
|---|---|
| oncoReveal® Essential LBx Panel (24 reactions) | HLA-HS-1006-24 |
| Pillar Unique Dual Index Kit Options | Reactions | Part number |
|---|---|---|
| Pillar Biosciences LBx Indexing Kit A | 24 Combinations, 96 reactions | IDX-PI-1013-96 |
| Pillar Biosciences LBx Indexing Kit B | 24 Combinations, 96 reactions | IDX-PI-1014-96 |