Simplifying
NGS Workflows.
Delivering Rapid,
Accurate Results.

At Pillar Biosciences, we develop precision research-use-only (RUO) and IVD diagnostic kitted products with our proprietary technologies designed to simplify next-generation sequencing (NGS) workflows while delivering robust, and accurate genomic results. Our platform of technologies —SLIMamp®, PiVAT®, and VersaTile™— work together to empower laboratories to achieve rapid, reliable results, driving actionable insights to support personalized patient care.

Improve Your Lab’s Efficiency and Flexibility

Single tube, one-day, fully automatable workflow drives more accurate, timely and cost-effective results to get patients on the right therapy faster.

Pillar’s oncoReveal® Workflow

Discover how Pillar’s oncoReveal workflow streamlines NGS-based tumor profiling with a single-tube, high-efficiency workflow. Seamless integration of SLIMamp® chemistry and Pivat® bioinformatics, enables labs to process samples to report within 48 hours. Designed for both research and diagnostic applications, the oncoReveal® workflow detects actionable biomarkers while minimizing laboratory hands-on time.

Scalable Testing
Less Tissue Required

  • Enables high-quality NGS results on low-input DNA samples*
  • High assay sensitivity on low-tumor content and degraded clinical samples

Efficient Workflow
Faster High Confidence Analysis

  • Single-tube workflow with 3.5
    hours of hands-on time
  • Multiple optional stopping points
    during workflow enables flexible
    sample batching
  • Sample to report in <48 hours

Powerful Bioinformatics Faster Clinical Results

  • Enables more sensitive and confident detection of clinically relevant genomic variants
  • Provides integrated clinical reporting for IVD products and tertiary analysis capabilities for select panels

*The term “low-input” refers to samples with <40ng of input DNA.

SLIMamp®: Streamlined NGS Library Preparation Technology

Our Stem-Loop Inhibition Mediated Amplification (SLIMamp®) technology enables highly multiplexed PCR in a single tube. By using tagged primers to form stem-loop structures, SLIMamp selectively blocks unwanted amplification, reducing hands-on time and minimizing errors. SLIMamp’s streamlined workflow improves NGS laboratory efficacy while maintaining high sensitivity for detecting clinically relevant variants, even at low allele frequencies.
Test-Tube
Rapid, single-tube workflow: Simplifies library prep to 3 hours of hands-on time
DNA
High confidence calls with low DNA input: Detects clinically relevant variants at <2.5% allele frequency with as little as 2.5ng of input DNA1
automatable
Fully automatable: Streamlined NGS workflow easily automated on multiple platforms
1. Reference notation goes here

PiVAT®:
Precision Bioinformatics

The Pillar Variant Analysis Toolkit (PiVAT) is Pillar’s robust secondary bioinformatics pipeline designed for high-confidence genomic analysis.

Workflow
Platform-agnostic: Compatible with major NGS instruments and workflows, enhancing genomic reporting efficiency for solid tumors and liquid biopsies alike
generation
Rapid turnaround: Supports the generation of research and clinical reports in hours, not days to support faster genomic profiling and patient management
Certified
Clinical-grade accuracy: Optimized for precision medicine applications, with variant calling down to 1% allele frequency, enables rapid analysis of genomic data to actionable insights

VersaTile™: Optimized
Panel Design

VersaTile™ helps revolutionize NGS panel development with intelligent primer design that combines advanced algorithms and cluster-based computing. VersaTile’s machine-learning backbone enables proactive error prevention, reducing off-target effects while maintaining optimal coverage across coding regions, fusion hotspots, and immunotherapy biomarkers. This precision makes VersaTile ideal for developing panels that require rapid iteration without sacrificing clinical-grade reliability.

This AI-driven platform meticulously evaluates key region of interest, automatically masking unsuitable primer sites to ensure optimal amplification efficiency and NGS panel design.

generation
Comprehensive primer generation: Systematically designs all possible primer sets for each target region, eliminating manual curation bottlenecks and accelerating panel design
Computer
High-performance computing: Leverages cluster-based analysis to calculate billions of primer interactions, identifying the most viable tiling solutions for challenging genomic regions and complex gene panels
optimization
Adaptive optimization: Dynamically balances primer characteristics (GC content, Tm, specificity) to create high-yield primer pools tailored to specific assay requirements

Empowering Laboratories, Accelerating NGS Results

Pillar’s technologies bridge the gap between research and clinical practice, enabling labs to adopt NGS with confidence. Our tools ensure precision, scalability, and accessibility—critical for advancing Decision Medicine™.

Pillar’s NGS Technology
Platform Video

Watch how Pillar’s integrated technologies transform complex genomic data into clinical insights using SLIMamp®, PiVAT®, and VersaTile™ to collectively deliver end-to-end solutions—from prep to analysis— all while being optimized for accuracy and speed. >>>

Interested in optimizing
your NGS workflow?

Contact us to explore how Pillar’s solutions can meet your lab’s needs.

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