MRDTect™
NPM1 and FLT3 ITD Panel
The MRDTect NPM1 and FLT3 ITD Panel is a robust NGS assay designed to interrogate low allele frequency NPM1 mutations and FLT3 internal tandem duplications (ITDs). The panel uses proprietary Stem-Loop Inhibition-Mediated amplification (SLIMamp®) technology, a tiled amplicon-based library prep chemistry, designed by Pillar’s AI-empowered VersaTile™ Primer Design tool, for efficient single-tube target enrichment.
MRDTect NPM1 and FLT3 ITD Panel
| FLT3 | NPM1 |
| FLT3 | NPM1 |
Simple, Rapid NGS Library Prep Workflow
- Single-tube library prep simplifies assay setup and reduces hands-on time
- Flexible stopping points support efficient sample batching
- Faster turnaround times with sample-to-result in <48 hours
Accurate, Reproducible Results
- Variants detectable at VAFs* as low as 10-5
- Consistent performance across low-input and degraded DNA samples
- Robust assay design supports reproducible results across runs
Scalable NGS Testing with Intuitive Informatics
- Facilitates an efficient and economic adoption of in-house NGS workflows
- Low read requirements support scalable, high-throughput testing
- Integrated informatics with a user-friendly Graphical User Interface (GUI)
- Streamlined analysis supports a clear path from sample to results
*VAF (variant allele frequency) detection level is dependent on input amount and number of reads
For Research Use Only. Not for use in diagnostic procedures.
Panel Specifications
| Enrichment chemistry | Amplicon-based multiplex PCR |
| Number of pools | 1 pool |
| Number of genes | 2 |
| Total panel size | 0.4kb |
| Variant types | SNVs, indels, small duplications, internal tandem duplications (ITDs) |
| Average amplicon size | 74bp |
| Recommended DNA input range | For a target VAF of 10-4: 500ng to 700ng For a target VAF of 10-5: 700ng |
| Sample types | DNA from blood, PBMCs, WBCs, or bone marrow |
| Mapping rate** | ~99.6% |
| % on-target aligned reads** | ~99.2% |
| Recommended Reads Per Sample† | For a target VAF of 10-4: ≥1.2M paired-end reads For a target VAF of 10-5: ≥8.9M paired-end reads |
**Mapping rate and percentage of on-target aligned reads are averages based on internal testing performed using reference standard materials and analysis using PiVAT®. Metrics are calculated using results from 500-700ng input. †Recommended reads per sample depends on input amount and targeted minimum VAF.
Ordering Information
Select the panel and one of the index kit options listed below.
| Panel | Part Number |
|---|---|
| MRDTect NPM1 and FLT3 ITD (24 reactions) | HDA-MY-1011-24 |
| Pillar Index Kit Options | Reactions | Part Number |
|---|---|---|
| Pillar Biosciences LBx Indexing Kit A | 24 Combinations, 96 Reactions | IDX-PI-1013-96 |
| Pillar Biosciences LBx Indexing Kit B | 24 Combinations, 96 Reactions | IDX-PI-1014-96 |