oncoReveal®
BRCA1 & BRCA2 plus CNV Panel
The oncoReveal® BRCA1 & BRCA2 plus CNV Panel is designed for the detection of exon-level copy number variation (CNV) across the entire coding region of the BRCA1 and BRCA2 genes. The assay is designed with shorter amplicons to allow for detection of mutations from FFPE samples, while also being compatible with intact genomic DNA from blood samples. The assay sequences the full coding region plus 10bp of flanking introns. The panel uses proprietary Stem-Loop Inhibition-Mediated amplification (SLIMamp®) technology, a tiled amplicon-based library prep chemistry, designed by Pillar’s AI-empowered VersaTile™ Primer Design tool, for efficient single-tube target enrichment.
Panel Specifications*
| Enrichment chemistry | Multiplex PCR using tiled amplicons |
| Number of pools | 1 pool |
| Number of genes/amplicons | 34/201 |
| Number of targets | Hotspots in 33 genes; full CDS coverage of TP53; 24 MSI sites; CNAs in 3 genes |
| Variant types | SNVs, indels, CNAs, and MSI |
| Average amplicon size | 90bp |
| Recommended DNA input range | 10ng to 30ng |
| Sample types | cfDNA from plasma |
| Mapping rate | ≥90% |
| % on-target aligned reads | ≥85% |
| Coverage uniformity | |
| (% targets with >0.2X mean coverage) | ≥90% |
| Recommended Reads Per Sample | ~10 million paired-end reads |
| Total assay time (from DNA to sequencer) | <8 hours |
Simple NGS Library Prep Workflow
Maintain control of samples and results with single-tube, tiled amplification that can be performed in-house by any NGS labSensitive and Robust Chemistry
Achieve variant detection as low as 1% VAF* even with limited DNA input or poor sample qualityReduced Fully-Loaded Lab Costs
Improve lab efficiency and reduce “no calls”, repeat testing, and difficult interpretation decisions*VAF, variant allele frequency
For Research Use Only. Not for use in diagnostic procedures..
Ordering Information
Select the panel AND one of the index kit options listed below.
| Panel | Part number |
|---|---|
| oncoReveal® Essential LBx Panel (24 reactions) | HLA-HS-1006-24 |
| Pillar Unique Dual Index Kit Options | Reactions | Part number |
|---|---|---|
| Pillar Biosciences LBx Indexing Kit A | 24 Combinations, 96 reactions | IDX-PI-1013-96 |
| Pillar Biosciences LBx Indexing Kit B | 24 Combinations, 96 reactions | IDX-PI-1014-96 |