oncoReveal®
Core LBx Panel

The oncoReveal® Core LBx panel is a robust NGS assay that interrogates 104 genes of interest across multiple solid tumor cancer types. The panel is specifically designed for cell-free DNA (cfDNA) extracted from plasma and can detect four types of variants: single nucleotide variants (SNVs), small insertion/deletion (indel) variants, copy number amplification (CNA), and microsatellite instability (MSI). The panel uses proprietary Stem-Loop Inhibition-Mediated amplification (SLIMamp®) technology, a tiled amplicon-based library prep chemistry, designed by Pillar’s AI-empowered Versatile™ Primer Design tool, for efficient single-tube target enrichment.

oncoReveal® Core LBx Panel (104 genes)

AKT1AXIN2CDKN2AEZH2GNAQKRASMTORPAK7PTPRDROS1TP53
ALKAXLCICFBXW7GNAS▲ MAP2K1MYCPDCD1PTPRSRUNX1TSC1
APCB2MCREBBP▲ FGFR1HNF1AMAP2K2MYOD1▲ PDGFRARAC1SF3B1U2AF1
ARBCORCTCF▲ FGFR2HRASMAPK1NCOR1▲ PIK3CARB1SMAD4VHL
ARAFBRAFCTNNB1▲ FGFR3IDH1MED12NF1PIK3R1RETSOX9
ARID1ACARD11▲ EGFRFLCNIDH2▲ METNFE2L2POLERAF1SPOP
ARID2CCND1EP300FOXL2IKZF1MLH1NOTCH1PPP2R1ARHEBSTAT5B
ASXL1CDH1▲ ERBB2GATA3JAK1MLL2NRASPTCH1RHOASMO
ATMCDK4ERBB3GLI1KDM5AMRE11ANTRK1PTENRIT1STK11
ATRXCDK6ESR1GNA11▲ KITMSH6NTRK3PTPN11RNF43TCF7L2
AKT1EZH2MTORROS1
ALKFBXW7MYCRUNX1
APC▲ FGFR1MYOD1SF3B1
AR▲ FGFR2NCOR1SMAD4
ARAF▲ FGFR3NF1SOX9
ARID1AFLCNNFE2L2SPOP
ARID2FOXL2NOTCH1STAT5B
ASXL1GATA3NRASSMO
ATMGLI1NTRK1STK11
ATRXGNA11NTRK3TCF7L2
AXIN2GNAQPAK7TP53
AXLGNASPDCD1TSC1
B2MHNF1A▲ PDGFRAU2AF1
BCORHRAS▲ PIK3CAVHL
BRAFIDH1PIK3R1
CARD11IDH2POLE
CCND1IKZF1PPP2R1A
CDH1JAK1PTCH1
CDK4KDM5APTEN
CDK6▲ KITPTPN11
CDKN2AKRASPTPRD
CIC▲ MAP2K1PTPRS
CREBBPMAP2K2RAC1
CTCFMAPK1RB1
CTNNB1MED12RET
▲ EGFR▲ METRAF1
EP300MLH1RHEB
▲ ERBB2MLL2RHOA
ERBB3MRE11ARIT1
ESR1MSH6RNF43

Copy Number Amplifications (CNAs) can also be detected in genes indicated by ▲.
Genes marked in green indicate full CDS coverage

Simple NGS Library Prep Workflow

Maintain control of samples and results with single-tube, tiled amplification that can be performed in-house by any NGS lab

Sensitive and Robust Chemistry

Achieve variant detection as low as 1% VAF* even with limited DNA input or poor sample quality

Reduced Fully-Loaded Lab Costs

Improve lab efficiency and reduce “no calls”, repeat testing, and difficult interpretation decisions

*VAF, variant allele frequency
For Research Use Only. Not for use in diagnostic procedures..

Panel Specifications*

Enrichment chemistryMultiplex PCR using tiled amplicons
Number of pools1 pool
Number of genes/amplicons104/446
Number of targetsHotspots in 103 genes; full CDS coverage of TP53; CNAs in 10 genes; 12.9kb total panel size
Variant typesSNVs, indels, CNAs, and MSI
Average amplicon size80bp
Recommended DNA input range10ng to 30ng
Sample typescfDNA from plasma
Mapping rate≥90%
% on-target aligned reads≥90%
Coverage uniformity
(% targets with >0.2X mean coverage)≥90%
Recommended Reads Per Sample~33 million paired-end reads
Total assay time (from DNA to sequencer)<10 hours

* Mapping rate, percentage of on-target aligned reads, and coverage uniformity metrics are based on internal testing performed using reference standard materials

Ordering Information

Select the panel AND one of the index kit options listed below.

PanelPart number
oncoReveal® Core LBx Panel (24 reactions)HLA-HS-1004-24
Pillar Unique Dual Index Kit OptionsReactionsPart number
Pillar Biosciences LBx Indexing Kit A24 Combinations, 96 reactionsIDX-PI-1013-96
Pillar Biosciences LBx Indexing Kit B24 Combinations, 96 reactionsIDX-PI-1014-96

User Guide

Core LBx Panel
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Product Sheet

Core LBx Panel
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