oncoReveal®
Core LBx Panel

The oncoReveal® Core LBx panel is a robust NGS assay that interrogates 104 genes of interest across multiple solid tumor cancer types. The panel is specifically designed for cell-free DNA (cfDNA) extracted from plasma and can detect four types of variants: single nucleotide variants (SNVs), small insertion/deletion (indel) variants, copy number amplification (CNA), and microsatellite instability (MSI). The panel uses proprietary Stem-Loop Inhibition-Mediated amplification (SLIMamp®) technology, a tiled amplicon-based library prep chemistry for efficient single-tube target enrichment.

oncoReveal® Core LBx Panel (104 genes)

AKT1AXIN2CDKN2AEZH2GNAQKRASMTORPAK7PTPRDROS1TP53
ALKAXLCICFBXW7GNAS▲ MAP2K1MYCPDCD1PTPRSRUNX1TSC1
APCB2MCREBBP▲ FGFR1HNF1AMAP2K2MYOD1▲ PDGFRARAC1SF3B1U2AF1
ARBCORCTCF▲ FGFR2HRASMAPK1NCOR1▲ PIK3CARB1SMAD4VHL
ARAFBRAFCTNNB1▲ FGFR3IDH1MED12NF1PIK3R1RETSOX9
ARID1ACARD11▲ EGFRFLCNIDH2▲ METNFE2L2POLERAF1SPOP
ARID2CCND1EP300FOXL2IKZF1MLH1NOTCH1PPP2R1ARHEBSTAT5B
ASXL1CDH1▲ ERBB2GATA3JAK1MLL2NRASPTCH1RHOASMO
ATMCDK4ERBB3GLI1KDM5AMRE11ANTRK1PTENRIT1STK11
ATRXCDK6ESR1GNA11▲ KITMSH6NTRK3PTPN11RNF43TCF7L2
AKT1EZH2MTORROS1
ALKFBXW7MYCRUNX1
APC▲ FGFR1MYOD1SF3B1
AR▲ FGFR2NCOR1SMAD4
ARAF▲ FGFR3NF1SOX9
ARID1AFLCNNFE2L2SPOP
ARID2FOXL2NOTCH1STAT5B
ASXL1GATA3NRASSMO
ATMGLI1NTRK1STK11
ATRXGNA11NTRK3TCF7L2
AXIN2GNAQPAK7TP53
AXLGNASPDCD1TSC1
B2MHNF1A▲ PDGFRAU2AF1
BCORHRAS▲ PIK3CAVHL
BRAFIDH1PIK3R1
CARD11IDH2POLE
CCND1IKZF1PPP2R1A
CDH1JAK1PTCH1
CDK4KDM5APTEN
CDK6▲ KITPTPN11
CDKN2AKRASPTPRD
CIC▲ MAP2K1PTPRS
CREBBPMAP2K2RAC1
CTCFMAPK1RB1
CTNNB1MED12RET
▲ EGFR▲ METRAF1
EP300MLH1RHEB
▲ ERBB2MLL2RHOA
ERBB3MRE11ARIT1
ESR1MSH6RNF43

Copy Number Amplifications (CNAs) can also be detected in genes indicated by ▲.
Genes marked in green indicate full CDS coverage

Simple, Rapid NGS Library Prep Workflow

  • Single-tube library prep simplifies assay setup and reduces hands-on time
  • Flexible stopping points support efficient sample batching
  • Faster turnaround times with sample-to-result in <48 hours

Accurate, Reproducible Results

  • Detect variants down to 0.1% VAF*
  • Consistent performance across low-input and degraded DNA samples
  • Robust assay design supports reproducible results across runs

Scalable NGS Testing with Intuitive Informatics

  • Facilitates an efficient and economic adoption of in-house NGS workflows
  • Low read requirements support scalable, high-throughput testing
  • Integrated informatics with user-friendly GUI
  • Streamlined analysis supports a clear path from sample to results

*VAF, variant allele frequency; VAF detection may be supported at lower frequency for key variants
For Research Use Only. Not for use in diagnostic procedures.

Panel Specifications*

Enrichment chemistryMultiplex PCR using tiled amplicons
Number of pools1 pool
Number of genes | amplicons104 | 447
Total panel size13.9kb
Variant typesSNVs, indels, CNAs, and MSI
Average amplicon size80bp
Recommended DNA input range10ng to 30ng
Sample typescfDNA from plasma
Mapping rate~90%
% on-target aligned reads~90%
Coverage uniformity
(% targets with >0.2X mean coverage)		~90%
Recommended Reads Per Sample~33 million paired-end reads
Total assay time (from DNA to sequencer)<10 hours

* Mapping rate, percentage of on-target aligned reads, and coverage uniformity metrics are averages based on internal testing performed using reference standard materials analysis using PiVAT®

Ordering Information

Select the panel AND one of the index kit options listed below.

PanelPart number
oncoReveal® Core LBx Panel (24 reactions)HLA-HS-1004-24
Pillar Unique Dual Index Kit OptionsReactionsPart number
Pillar Biosciences LBx Indexing Kit A24 Combinations, 96 reactionsIDX-PI-1013-96
Pillar Biosciences LBx Indexing Kit B24 Combinations, 96 reactionsIDX-PI-1014-96

User Guide

Core LBx Panel

 

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Product Sheet

Core LBx Panel

 

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SDS Sheet

Core LBx Panel

 

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White Paper

Investigating the panel performance for hotspot ESR1 variants

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