oncoReveal®
Fusion LBx Panel
The oncoReveal® Fusion LBx panel is a robust NGS assay that interrogates multiple gene rearrangement regions of interest across multiple solid tumor cancer types. The panel uses proprietary Stem-Loop Inhibition-Mediated amplification (SLIMamp®) technology, a tiled amplicon-based library prep chemistry, designed by Pillar’s AI-empowered VersaTile™ Primer Design tool, for efficient single-tube target enrichment.
| Target Driver Genes | Fusion Partners |
|---|---|
| ALK | EML4, CLTC, HIP1, KIF5B, KLC1, STRN, TFG, TPM3, TPR, MSN |
| BRAF | KIAA1549, MKRN1, FAM131B, AKAP9 |
| EGFR | SEPT14, PSPH, RAD51 |
| ERG | TMPRSS2 |
| FGFR2 | BICC1, CASP7 |
| FGFR3 | TACC3, BAIAP2L1 |
| MET | KIF5B |
| NRG1 | CD74, SLC3A2, VAMP2 |
| NTRK1 | TPM3, TFG, LMNA, SQSTM1, CHTOP, ARHGEF2, NFASC, IRF2BP2, PPL, BCAN, SCYL3, TP53, CD74, MPRIP, TPR |
| NTRK2 | STRN, AFAP1, NACC2, BCR, TRIM24, QKI, PAN3, SQSTM1 |
| NTRK3 | ETV6, BTBD1, EML4, SQSTM1, TFG, RBPMS |
| PBX1 | TCF3 |
| PPARG | PAX8, CREB3L2 |
| PRKACA | DNAJB1 |
| RAF1 | ESRP1, SRGAP3 |
| RET | CCDC6, CUX1, KIF5B, NCOA4, TRIM33, PRKAR1A |
| ROS1 | CCDC6, CD74, CLTC, EZR, GOPC, LRIG3, MSN, SDC4, SLC34A2, TFG, TPM3 |
| TFE3 | SFPQ, ASPSCR1, CLTC, PRCC, NONO |
| MET exon 14 skipping, EGFR variant III; expression control genes: PSMB2 & PUM1 |
| ABL1 | DDX41 | KDM6A | RUNX1 |
| ANKRD26 | DNMT3A | KIT | SETBP1 |
| ASXL1 | ETNK1 | KMT2A | SF3B1 |
| ATRX | ETV6 | KRAS | SMC1A |
| BCOR | EZH2 | MPL | SMC3 |
| BCORL1 | FLT3 | NF1 | SRSF2 |
| BRAF | GATA1 | NPM1 | STAG1 |
| CALR | GATA2 | NRAS | STAG2 |
| CBL | GNAS | PDGFRA | TET2 |
| CBLB | HRAS | PHF6 | TP53 |
| CBLC | IDH1 | PIGA | U2AF1 |
| CDKN2A | IDH2 | PPM1D | WT1 |
| CEBPA | IKZF1 | PTEN | ZRSR2 |
| CSF3R | JAK2 | PTPN11 | |
| CUX1 | JAK3 | RAD21 |
Simple, Rapid NGS Library
Prep Workflow
- Single-tube library prep simplifies assay setup and reduces hands-on time
- Flexible stopping points support efficient sample batching
Accurate, Reproducible Results
- Consistent performance across low-input and degraded RNA samples
- Robust assay design supports reproducible results across runs
Scalable NGS Testing with Intuitive Informatics
- Low read requirements support scalable, high-throughput testing
- Integrated informatics with user-friendly GUI
For Research Use Only. Not for use in diagnostic procedures..
Panel Specifications*
| Enrichment chemistry | Multiplex PCR using tiled amplicons |
| Number of pools | 1 pool |
| Number of genes | partners | 18 genes | >80 partners, plus MET exon 14 skipping and EGFR variant III |
| Variant types | RNA fusion transcripts |
| Average amplicon size | 80bp |
| Recommended RNA input range | 10ng to 30ng |
| Sample types | cfRNA from plasma |
| Recommended reads per sample | 100,000 paired-end reads |
| Total assay time (from RNA to sequencer) | <10 hours (including reverse transcription) |
Ordering Information
Select the panel AND one of the index kit options listed below.
| Panel | Part number |
|---|---|
| oncoReveal® Fusion LBx Panel (24 reactions) | HLA-HS-1005-24 |
| Pillar Unique Dual Index Kit Options | Reactions | Part number |
|---|---|---|
| Pillar Biosciences LBx Indexing Kit A | 24 Combinations, 96 reactions | IDX-PI-1013-96 |
| Pillar Biosciences LBx Indexing Kit B | 24 Combinations, 96 reactions | IDX-PI-1014-96 |