oncoReveal®
HRD v2 Panel
The oncoReveal® HRD v2 Panel is designed for detection of SNVs, indels, and BRCA1 & BRCA2 exon level CNVs. 33 HRD-related genes can be assessed in either FFPE or blood samples. The panel uses proprietary Stem-Loop Inhibition-Mediated amplification (SLIMamp®) technology, a tiled amplicon-based library prep chemistry, designed by Pillar’s AI-empowered VersaTile™ Primer Design tool, for efficient single-tube target enrichment.
oncoReveal® HRD v2 Panel (33 genes)
| ARID1A | BRCA2 | ESR1 | FEN1 | PPP2R1A | RAD51D |
| ATM | BRIP1 | FANCA | KRAS | PTEN | RAD54L |
| ATR | CDK12 | FANCC | MRE11A | RAD50 | TP53 |
| BARD1 | CHEK2 | FANCD2 | NBN | RAD51 | |
| BRAF | CTNNB1 | FANCE | PALB2 | RAD51B | |
| BRCA1 | ERBB2 | FANCF | PIK3CA | RAD51C |
| ARID1A | ESR1 | PPP2R1A |
| ATM | FANCA | PTEN |
| ATR | FANCC | RAD50 |
| BARD1 | FANCD2 | RAD51 |
| BRAF | FANCE | RAD51B |
| BRCA1 | FANCF | RAD51C |
| BRCA2 | FEN1 | RAD51D |
| BRIP1 | KRAS | RAD54L |
| CDK12 | MRE11A | TP53 |
| CHEK2 | NBN | |
| CTNNB1 | PALB2 | |
| ERBB2 | PIK3CA |
Genes marked in green indicate full CDS coverage
Simple NGS Library Prep Workflow
Maintain control of samples and results with single-tube, tiled amplification that can be performed in-house by any NGS labSensitive and Robust Chemistry
Achieve variant detection as low as 1% VAF* even with limited DNA input or poor sample qualityReduced Fully-Loaded Lab Costs
Improve lab efficiency and reduce “no calls”, repeat testing, and difficult interpretation decisions*VAF, variant allele frequency
For Research Use Only. Not for use in diagnostic procedures..
Panel Specifications*
| Enrichment chemistry | Multiplex PCR using tiled amplicons |
| Number of pools | 1 pool |
| Number of genes/amplicons | 33/1,314 |
| Number of targets | Full CDS coverage and exon-level CNVs for BRCA1 & BRCA2 genes, hotspots in 31 additional |
| HRD-related genes; 114.5kb total size | |
| Variant types | SNVs, indels, exon level CNVs for BRCA1 & BRCA2 |
| Average amplicon size | 157bp |
| Recommended DNA input range | 20ng to 80ng |
| Sample types | DNA from tissue, blood, or FFPE |
| Mapping rate | 97.9% ± 1.7% |
| % on-target aligned reads | 91.1% ± 0.8% |
| Coverage uniformity | |
| (% targets with >0.2X mean coverage) | 92.5% ± 0.9% |
| Recommended Reads Per Sample | ~6.5 million to 7.5 million paired-end reads |
| Total assay time (from DNA to sequencer) | <8 hours |
*Mapping rate, percentage of on-target aligned reads, and coverage uniformity metrics are based on internal testing performed using reference standard materials
Ordering Information
Select the panel AND one of the index kit options listed below.
| Panel | Part number |
|---|---|
| oncoReveal® HRD v2 Panel (24 reactions) | HDA-HR-1002-24 |
| Pillar Index Kit options | Reactions | Part number |
|---|---|---|
| Pillar Custom Index Primers Kit A | 32 Combinations, 96 reactions | IDX-PI-1001-96 |
| Pillar Custom Index Primers Kit D | 96 Combinations, 192 reactions | IDX-PI-1004-192 |