oncoReveal®
MGMT & MLH1 Methylation Panel
The oncoReveal® MGMT & MLH1 Methylation Panel is a robust NGS assay that interrogates CpG sites within MGMT and MLH1 that have undergone bisulfite conversion for methylome analysis. The panel uses proprietary Stem-Loop Inhibition-Mediated amplification (SLIMamp®) technology, a tiled amplicon-based library prep chemistry, designed by Pillar’s AI-empowered VersaTile™ Primer Design tool, for efficient single-tube target enrichment.
Panel Specifications*
| Enrichment chemistry | Multiplex PCR using tiled amplicons |
| Number of pools | 1 pool |
| Number of genes/amplicons | 2/5 |
| Variant types | Methylation status of >60 CpG sites |
| Average amplicon size | 127bp |
| Recommended DNA input range | 5ng to 20ng |
| Sample types | Bisulfite or enzymatically converted DNA |
| Mapping rate | 90.00% |
| % on-target aligned reads | 80.00% |
| Coverage uniformity | 90.00% |
| (% targets with >0.2X mean coverage) | |
| Recommended reads per sample | ~24,000 paired-end reads |
| Total assay time (from DNA to sequencer) | <5 hours |
*Mapping rate, percentage of on-target aligned reads, and coverage uniformity metrics are based on internal testing performed using reference standard materials
Simple NGS Library Prep Workflow
Maintain control of samples and results with single-tube, tiled amplification that can be performed in-house by any NGS labSensitive and Robust Chemistry
Achieve variant detection as low as 1% VAF* even with limited DNA input or poor sample qualityReduced Fully-Loaded Lab Costs
Improve lab efficiency and reduce “no calls”, repeat testing, and difficult interpretation decisions*VAF, variant allele frequency
For Research Use Only. Not for use in diagnostic procedures..
Ordering Information
Select the panel AND one of the index kit options listed below.
| Panel | Part number |
|---|---|
| oncoReveal® MGMT & MLH1 Methylation Panel (24 reactions) | HDA-HR-1010-24 |
| Pillar Index Kit options | Reactions | Part number |
|---|---|---|
| Pillar Custom Index Primers Kit A | 32 Combinations, 96 reactions | IDX-PI-1001-96 |
| Pillar Custom Index Primers Kit D | 96 Combinations, 192 reactions | IDX-PI-1004-192 |