oncoReveal®
Multi-Cancer RNA Fusion v2 Panel
The oncoReveal® Multi-Cancer RNA Fusion v2 Panel is a robust NGS assay that interrogates multiple gene rearrangement regions of interest* across multiple solid tumor cancer types. The panel uses proprietary Stem-Loop Inhibition-Mediated amplification (SLIMamp®) technology, a tiled amplicon-based library prep chemistry, designed by Pillar’s AI-empowered VersaTile™ Primer Design tool, for efficient single-tube target enrichment.
| Driver Genes | Fusion Partners |
|---|---|
| ALK | EML4, CLTC, HIP1, KIF5B, KLC1, STRN, TFG, TPM3, TPR, MSN |
| BRAF | KIAA1549, MKRN1, FAM131B, AKAP9 |
| EGFR | SEPT14, PSPH, RAD51 |
| ERG | TMPRSS2 |
| FGFR2 | BICC1, CASP7 |
| FGFR3 | TACC3, BAIAP2L1 |
| MET | KIF5B |
| NRG1 | CD74, SLC3A2, VAMP2 |
| NTRK1 | TPM3, TFG, LMNA, SQSTM1, CHTOP, ARHGEF2, NFASC, IRF2BP2, PPL, BCAN, SCYL3, TP53, CD74, MPRIP, TPR |
| NTRK2 | AFAP1, NACC2, BCR, TRIM24, QKI, PAN3, SQSTM1, STRN |
| NTRK3 | ETV6, BTBD1, EML4, SQSTM1, TFG, RBPMS |
| PBX1 | TCF3 |
| PPARG | PAX8, CREB3L2 |
| PRKACA | DNAJB1 |
| RAF1 | ESRP1, SRGAP3 |
| RET | CCDC6, CUX1, KIF5B, NCOA4, TRIM33, PRKAR1A |
| ROS1 | CCDC6, CD74, CLTC, EZR, GOPC, LRIG3, MSN, SDC4, SLC34A2, TFG, TPM3 |
| TFE3 | SFPQ, ASPSCR1, CLTC, PRCC, NONO |
| MET exon 14 skipping, EGFR variant III |
| 3’/5’ Expression Imbalance Ration Assessed |
|---|
| ALK ROS1 RET NTRK1 NTRK2 NTRK3 FGFR3 NRG1 PBX1 |
| Expression Control Genes |
|---|
| HMBS TBP |
| ABL1 | DDX41 | KDM6A | RUNX1 |
| ANKRD26 | DNMT3A | KIT | SETBP1 |
| ASXL1 | ETNK1 | KMT2A | SF3B1 |
| ATRX | ETV6 | KRAS | SMC1A |
| BCOR | EZH2 | MPL | SMC3 |
| BCORL1 | FLT3 | NF1 | SRSF2 |
| BRAF | GATA1 | NPM1 | STAG1 |
| CALR | GATA2 | NRAS | STAG2 |
| CBL | GNAS | PDGFRA | TET2 |
| CBLB | HRAS | PHF6 | TP53 |
| CBLC | IDH1 | PIGA | U2AF1 |
| CDKN2A | IDH2 | PPM1D | WT1 |
| CEBPA | IKZF1 | PTEN | ZRSR2 |
| CSF3R | JAK2 | PTPN11 | |
| CUX1 | JAK3 | RAD21 |
Simple NGS Library Prep Workflow
Maintain control of samples and results with single-tube, tiled amplification that can be performed in-house by any NGS labSensitive and Robust Chemistry
Achieve variant detection as low as 1% VAF* even with limited DNA input or poor sample qualityReduced Fully-Loaded Lab Costs
Improve lab efficiency and reduce “no calls”, repeat testing, and difficult interpretation decisionsFor Research Use Only. Not for use in diagnostic procedures..
Panel Specifications*
| Enrichment chemistry | Multiplex PCR using tiled amplicons |
| Number of pools | 1 pool |
| Number of genes/partners | 18 genes and >80 partners, plus MET exon 14 skipping |
| Variant types | Fusion RNA transcripts |
| Average amplicon size | 120bp |
| Recommended RNA input range | 20ng - 100ng RNA |
| Sample types | RNA from FFPE or tissue; cfRNA |
| Recommended reads per sample | ~50,000 paired-end reads |
| Total assay time (from RNA to sequencer) | <9 hours |
*Mapping rate, percentage of on-target aligned reads, and coverage uniformity metrics are based on internal testing performed using reference standard materials
Ordering Information
Select the panel AND one of the index kit options listed below.
| Panel | Part number |
|---|---|
| oncoReveal® Multi-Cancer RNA Fusion v2 Panel (24 reactions) | HRA-HS-1002-24 |
| Pillar Index Kit options | Reactions | Part number |
|---|---|---|
| Pillar Custom Index Primers Kit A | 32 Combinations, 96 reactions | IDX-PI-1001-96 |
| Pillar Custom Index Primers Kit D | 96 Combinations, 192 reactions | IDX-PI-1004-192 |
User Guide
Multi-Cancer RNA Fusion v2 Panel
Product Sheet
Multi-Cancer RNA Fusion v2 Panel