oncoReveal®
Multi-Cancer RNA Fusion v2 Panel
The oncoReveal® Multi-Cancer RNA Fusion v2 Panel is a robust NGS assay that interrogates multiple gene rearrangement regions of interest* across multiple solid tumor cancer types. The panel uses proprietary Stem-Loop Inhibition-Mediated amplification (SLIMamp®) technology, a tiled amplicon-based library prep chemistry, designed by Pillar’s AI-empowered VersaTile™ Primer Design tool, for efficient single-tube target enrichment.
| Driver Genes | Fusion Partners |
|---|---|
| ALK | EML4, CLTC, HIP1, KIF5B, KLC1, STRN, TFG, TPM3, TPR, MSN |
| BRAF | KIAA1549, MKRN1, FAM131B, AKAP9 |
| EGFR | SEPT14, PSPH, RAD51 |
| ERG | TMPRSS2 |
| FGFR2 | BICC1, CASP7 |
| FGFR3 | TACC3, BAIAP2L1 |
| MET | KIF5B |
| NRG1 | CD74, SLC3A2, VAMP2 |
| NTRK1 | TPM3, TFG, LMNA, SQSTM1, CHTOP, ARHGEF2, NFASC, IRF2BP2, PPL, BCAN, SCYL3, TP53, CD74, MPRIP, TPR |
| NTRK2 | AFAP1, NACC2, BCR, TRIM24, QKI, PAN3, SQSTM1, STRN |
| NTRK3 | ETV6, BTBD1, EML4, SQSTM1, TFG, RBPMS |
| PBX1 | TCF3 |
| PPARG | PAX8, CREB3L2 |
| PRKACA | DNAJB1 |
| RAF1 | ESRP1, SRGAP3 |
| RET | CCDC6, CUX1, KIF5B, NCOA4, TRIM33, PRKAR1A |
| ROS1 | CCDC6, CD74, CLTC, EZR, GOPC, LRIG3, MSN, SDC4, SLC34A2, TFG, TPM3 |
| TFE3 | SFPQ, ASPSCR1, CLTC, PRCC, NONO |
| MET exon 14 skipping, EGFR variant III |
| 3’/5’ Expression Imbalance Ratio Assessed |
|---|
| ALK ROS1 RET NTRK1 NTRK2 NTRK3 FGFR3 NRG1 PBX1 |
| Expression Control Genes |
|---|
| HMBS TBP |
| ABL1 | DDX41 | KDM6A | RUNX1 |
| ANKRD26 | DNMT3A | KIT | SETBP1 |
| ASXL1 | ETNK1 | KMT2A | SF3B1 |
| ATRX | ETV6 | KRAS | SMC1A |
| BCOR | EZH2 | MPL | SMC3 |
| BCORL1 | FLT3 | NF1 | SRSF2 |
| BRAF | GATA1 | NPM1 | STAG1 |
| CALR | GATA2 | NRAS | STAG2 |
| CBL | GNAS | PDGFRA | TET2 |
| CBLB | HRAS | PHF6 | TP53 |
| CBLC | IDH1 | PIGA | U2AF1 |
| CDKN2A | IDH2 | PPM1D | WT1 |
| CEBPA | IKZF1 | PTEN | ZRSR2 |
| CSF3R | JAK2 | PTPN11 | |
| CUX1 | JAK3 | RAD21 |
Simple, Rapid NGS Library Prep Workflow
- Single-tube library prep simplifies assay setup and reduces hands on time
- Flexible stopping points support efficient sample batching
Accurate, Reproducible Results
- Consistent performance across low-input and degraded RNA samples
- Robust assay design supports reproducible results across runs
Scalable NGS Testing with Intuitive Informatics
- Low read requirements support scalable, high throughput testing
- Integrated informatics with a user-friendly GUI
For Research Use Only. Not for use in diagnostic procedures..
Panel Specifications*
| Enrichment chemistry | Multiplex PCR using tiled amplicons |
| Number of pools | 1 pool |
| Number of genes | partners | 18 genes | >80 partners, plus MET exon 14 skipping EGFR variant III |
| Variant types | Fusion RNA transcripts |
| Average amplicon size | 120bp |
| Recommended RNA input rage | 25ng to 100ng RNA |
| Sample types | RNA from FFPE or tissue |
| Recommended reads per sample | ~100,000 paired-end reads |
| Total assay time (from RNA to sequencer) | <11 hours (including reverse transcription) |
*Mapping rate, percentage of on-target aligned reads, and coverage uniformity metrics are based on internal testing performed using reference standard materials
Ordering Information
Select the panel AND one of the index kit options listed below.
| Panel | Part number |
|---|---|
| oncoReveal® Multi-Cancer RNA Fusion v2 Panel (24 reactions) | HRA-HS-1002-24 |
| Pillar Index Kit options | Reactions | Part number |
|---|---|---|
| Pillar Custom Index Primers Kit A | 32 Combinations, 96 reactions | IDX-PI-1001-96 |
| Pillar Custom Index Primers Kit D | 96 Combinations, 192 reactions | IDX-PI-1004-192 |
User Guide
Multi-Cancer RNA Fusion v2 Panel
Product Sheet
Multi-Cancer RNA Fusion v2 Panel