oncoReveal®
Multi-Cancer with CNV & RNA Fusion Panel

The oncoReveal® Multi-Cancer with CNV & RNA Fusion Panel is a combined DNA/RNA multi-cancer panel. The assay combines the DNA-based oncoReveal® Multi-Cancer v4 with CNV Panel with the oncoReveal® Multi-Cancer RNA Fusion v2 Panel allowing for joint sequencing. The panel uses proprietary Stem-Loop Inhibition-Mediated amplification (SLIMamp®) technology, a tiled amplicon-based library prep chemistry, designed by Pillar’s AI-empowered VersaTile™ Primer Design tool, for efficient single-tube target enrichment.

Variants and CNVs detected from DNA

ABL1CDKN2AFBXW7GNASKITNPM1PTPN11SRC
AKTICSF1RFGFR1 HNF1AKRAS NRASRAC1STK11
ALKCTNNB1FGFR2 HRASMAP2K1 NTRK1RB1TP53
APCDDR2FGFR3 IDH1MET ◼ NTRK2RETVHL
ATMEGFR ◼ FLT3 IDH2MLH1NTRK3ROS1
BRAFERBB2 ◼ FOXL2JAK2MPLPDGFRA SMAD4
CCNE1 ERBB4GNA11JAK3MYC ◼ PIK3CA SMARCB1
CDH1EZH2GNAQKDR NOTCH1PTENSMO
ABL1FBXW7KITPTPN11
AKTIFGFR1 KRAS RAC1
ALKFGFR2 MAP2K1 RB1
APCFGFR3 MET ◼RET
ATMFLT3 MLH1ROS1
BRAFFOXL2MPLSMAD4
CCNE1 GNA11MYC ◼SMARCB1
CDH1GNAQNOTCH1SMO
CDKN2AGNASNPM1SRC
CSF1RHNF1ANRASSTK11
CTNNB1HRASNTRK1TP53
DDR2IDH1NTRK2VHL
EGFR ◼IDH2NTRK3
ERBB2 ◼JAK2PDGFRA
ERBB4JAK3PIK3CA
EZH2KDR PTEN
CNVs detected and verified by NIST reference standard are indicated by ◼ CNVs can also be detected in genes indicated by

Fusions and expression insights detected from RNA

Driver gene fusions (fusion partners not listed)

ALKEGFRFGFR2METNTRK1NTRK3PPARGRAF1
BRAFERGFGFR3NRG1NTRK2PBX1PRKACARET

3’/5’ Expression Imbalance Ratio Assessed

ALKFGFR3NRG1NTRK1NTRK2NTRK3PBX1RET

Expression Control Genes

HMBSTBP

Simple NGS Library Prep Workflow

Maintain control of samples and results with single-tube, tiled amplification that can be performed in-house by any NGS lab

Sensitive and Robust Chemistry

Achieve variant detection as low as 1% VAF* even with limited DNA input or poor sample quality

Reduced Fully-Loaded Lab Costs

Improve lab efficiency and reduce “no calls”, repeat testing, and difficult interpretation decisions

*VAF, variant allele frequency
For Research Use Only. Not for use in diagnostic procedures..

Panel Specifications*

oncoReveal® Multi-Cancer v4 with CNVoncoReveal® Multi-Cancer RNA Fusion v2
Enrichment chemistryMultiplex PCR using tiled ampliconsMultiplex PCR using tiled amplicons
Number of pools2 pools2 pools
Number of genes/amplicons60/34118 genes and >80 partners, plus MET exon 14 skipping
Number of targetsHotspots in 60 genes; CNVs for 14 genes;
~33.1kb total size		Fusions in 18 driver genes; expression for 11 genes
Variant typesSNVs, small and medium indels, and CNVsFusion RNA transcripts
Average amplicon size143bp120bp
Recommended input range5ng to 80ng DNA20ng to 100ng RNA
Sample typesDNA from tissue, blood; or FFPERNA from FFPE or tissue; cfRNA
Mapping rate99.23% ± 0.3%n/a
% on-target aligned reads99.5% ± 0.1%n/a
Coverage uniformity
(% targets with >0.2x mean coverage)		98.2% ± 0.7%n/a
Recommended reads per sample~1.4 million paired-end reads~50,000 paired-end reads per sample
Total assay time (from RNA to sequencer)<10.5 hours<10.5 hours
*Mapping rate, percentage of on-target aligned reads, and coverage uniformity metrics are based on internal testing performed using reference standard materials

Ordering Information

Select the panel and one of the index kit options listed below.

PanelPart number
oncoReveal® Multi-Cancer with CNV and RNA Fusion Panel (48 reactions)HNA-HS-1001-48
Pillar Index Kit optionsReactionsPart number
Pillar Custom Index Primers Kit A32 Combinations, 96 reactionsIDX-PI-1001-96
Pillar Custom Index Primers Kit D96 Combinations, 192 reactionsIDX-PI-1004-192

User Guide

Multi-Cancer with CNV & RNA Fusion Panel
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Product Sheet

Multi-Cancer with CNV & RNA Fusion Panel
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