oncoReveal®
Myeloid Panel
The oncoReveal® Myeloid Panel is a robust NGS assay that interrogates 58 genes of interest most relevant to myeloid cancer. The panel uses proprietary Stem-Loop Inhibition-Mediated amplification (SLIMamp®) technology, a tiled amplicon-based library prep chemistry for efficient single-tube target enrichment
oncoReveal® Myeloid Panel (58 genes)
| ABL1 | BRAF | CEBPA | ETV6 | HRAS | KDM6A | NPM1 | PTEN | SMC1A | TP53 |
| ANKRD26 | CALR | CSF3R | EZH2 | IDH1 | KIT | NRAS | PTPN11 | SMC3 | U2AF1 |
| ASXL1 | CBL | CUX1 | FLT3 | IDH2 | KMT2A | PDGFRA | RAD21 | SRSF2 | WT1 |
| ATRX | CBLB | DDX41 | GATA1 | IKZF1 | KRAS | PHF6 | RUNX1 | STAG1 | ZRSR2 |
| BCOR | CBLC | DNMT3A | GATA2 | JAK2 | MPL | PIGA | SETBP1 | STAG2 | |
| BCORL1 | CDKN2A | ETNK1 | GNAS | JAK3 | NF1 | PPM1D | SF3B1 | TET2 |
| ABL1 | DDX41 | KDM6A | RUNX1 |
| ANKRD26 | DNMT3A | KIT | SETBP1 |
| ASXL1 | ETNK1 | KMT2A | SF3B1 |
| ATRX | ETV6 | KRAS | SMC1A |
| BCOR | EZH2 | MPL | SMC3 |
| BCORL1 | FLT3 | NF1 | SRSF2 |
| BRAF | GATA1 | NPM1 | STAG1 |
| CALR | GATA2 | NRAS | STAG2 |
| CBL | GNAS | PDGFRA | TET2 |
| CBLB | HRAS | PHF6 | TP53 |
| CBLC | IDH1 | PIGA | U2AF1 |
| CDKN2A | IDH2 | PPM1D | WT1 |
| CEBPA | IKZF1 | PTEN | ZRSR2 |
| CSF3R | JAK2 | PTPN11 | |
| CUX1 | JAK3 | RAD21 |
Genes marked in green indicate full CDS coverage
Simple NGS Library Prep Workflow
Maintain control of samples and results with single-tube, tiled amplification that can be performed in-house by any NGS labSensitive and Robust Chemistry
Achieve variant detection as low as 1% VAF* even with limited DNA input or poor sample qualityReduced Fully-Loaded Lab Costs
Improve lab efficiency and reduce “no calls”, repeat testing, and difficult interpretation decisions*VAF, variant allele frequency
For Research Use Only. Not for use in diagnostic procedures..
Panel Specifications*
| Enrichment chemistry | Multiplex PCR using tiled amplicons |
| Number of pools | 1 pool |
| Number of genes/amplicons | 58/766 |
| Number of targets | Full CDS coverage of 18 genes, hotspots in 40 additional genes, FLT3 ITDs; 107.9kb total size |
| Variant types | SNVs, indels, ITD (internal tandem duplicates) |
| Average amplicon size | 217bp |
| Recommended DNA input range | 20ng to 60ng (20ng recommended) |
| Sample types | DNA from whole blood, PBMCs |
| Mapping rate | 99.6% ± 0.2% |
| % on-target aligned reads | 92.0% ± 5.3% |
| Coverage uniformity (% targets with >0.2X mean coverage) | 96.8% ± 1.0% |
| Recommended Reads Per Sample | ~4 million paired-end reads |
| Total assay time (from DNA to sequencer) | <8 hours |
*Mapping rate, percentage of on-target aligned reads, and coverage uniformity metrics are based on internal testing performed using reference standard materials
Ordering Information
Select the panel and one of the index kit options listed below.
| Panel | Part Number |
|---|---|
| oncoReveal® Myeloid Panel (24 reactions) | HDA-MY-1001-24 |
| Pillar Index Kit Options | Reactions | Part Number |
|---|---|---|
| Pillar Custom Index Primers Kit A | 32 Combinations, 96 Reactions | IDX-PI-1001-96 |
| Pillar Custom Index Primers Kit D | 96 Combinations, 192 Reactions | IDX-PI-1004-192 |