oncoReveal®
Myeloid v2 Panel
The oncoReveal® Myeloid v2 Panel is a robust NGS assay that interrogates 80 genes of interest most relevant to myeloid cancer. The panel uses proprietary Stem-Loop Inhibition-Mediated amplification (SLIMamp®) technology, a tiled amplicon-based library prep chemistry for efficient single-tube target enrichment
oncoReveal® Myeloid v2 Panel (80 genes)
| ABL1 | CALR | CSF3R | EZH2 | IDH2 | MAP2K1 | NPM1 | RAD21 | SMC1A | TET2 |
| ANKRD26 | CBL | CUX1 | FLT3 | IKZF1 | MBD4 | NRAS | RBBP6 | SMC3 | TP53 |
| ARAF | CBLB | DDX41 | GATA1 | JAK2 | MEN1 | PDGFRA | RUNX1 | SRSF2 | U2AF1 |
| ASXL1 | CBLC | DNMT3A | GATA2 | JAK3 | MPL | PHF6 | SAMD9 | STAG1 | U2AF2 |
| ATRX | CCND2 | ELANE | GNAS | KDM6A | MYC | PIGA | SAMD9L | STAG2 | UBA1 |
| BCOR | CDKN2A | ERCC6L2 | GNB1 | KIT | MYD88 | PPM1D | SETBP1 | STAT3 | UBTF |
| BCORL1 | CEBPA | ETNK1 | HRAS | KMT2A | NF1 | PTEN | SF3B1 | STAT5B | WT1 |
| BRAF | CHEK2 | ETV6 | IDH1 | KRAS | NOTCH1 | PTPN11 | SH2B3 | TERT (promoter) | ZRSR2 |
| ABL1 | DDX41 | KDM6A | RUNX1 |
| ANKRD26 | DNMT3A | KIT | SETBP1 |
| ASXL1 | ETNK1 | KMT2A | SF3B1 |
| ATRX | ETV6 | KRAS | SMC1A |
| BCOR | EZH2 | MPL | SMC3 |
| BCORL1 | FLT3 | NF1 | SRSF2 |
| BRAF | GATA1 | NPM1 | STAG1 |
| CALR | GATA2 | NRAS | STAG2 |
| CBL | GNAS | PDGFRA | TET2 |
| CBLB | HRAS | PHF6 | TP53 |
| CBLC | IDH1 | PIGA | U2AF1 |
| CDKN2A | IDH2 | PPM1D | WT1 |
| CEBPA | IKZF1 | PTEN | ZRSR2 |
| CSF3R | JAK2 | PTPN11 | |
| CUX1 | JAK3 | RAD21 |
Genes marked in green indicate full CDS coverage
Simple, Rapid NGS Library Prep Workflow
- Single-tube library prep simplifies assay setup and reduces hands-on time
- Flexible stopping points support efficient sample batching
- Faster turnaround times with sample-to-result in <48 hours
Accurate, Reproducible Results
- Detect variants down to 1% VAF*
- Consistent performance across low-input and degraded DNA samples
- Robust assay design supports reproducible results across runs
Scalable NGS Testing with Intuitive Informatics
- Facilitates an efficient and economic adoption of in-house NGS workflows
- Low read requirements support scalable, high-throughput testing
- Integrated informatics with a user-friendly Graphical User Interface (GUI)
- Streamlined analysis supports a clear path from sample to results
*VAF (variant allele frequency) listed is for select variants.
For Research Use Only. Not for use in diagnostic procedures.
Panel Specifications*
| Enrichment chemistry | Multiplex PCR using tiled amplicons |
| Number of pools | 1 pool |
| Number of genes | amplicons | 80 | 874 |
| Total panel size | 124.3kb |
| Variant types | SNVs, indels, ITD (internal tandem duplications) |
| Average amplicon size | 215bp |
| Recommended DNA input range | 20ng to 60ng |
| Sample types | DNA from whole blood or PBMCs |
| Mapping rate | ~98.9% |
| % on-target aligned reads | ~87.5% |
| Coverage uniformity (% targets with >0.2X mean coverage) | ~97.4% |
| Recommended Reads Per Sample | ~5.14 million paired-end reads |
| Total assay time (from DNA to sequencer) | <8.5 hours |
*Mapping rate, percentage of on-target aligned reads, and coverage uniformity metrics are averages based on internal testing performed using reference standard materials and analysis using PiVAT®
Ordering Information
Select the panel and one of the index kit options listed below.
| Panel | Part Number |
|---|---|
| oncoReveal® Myeloid v2 Panel (24 reactions) | HDA-MY-1010-24 |
| Pillar Index Kit Options | Reactions | Part Number |
|---|---|---|
| Pillar Custom Indexing Kit A | 32 Combinations, 96 Reactions | IDX-PI-1001-96 |
| Pillar Custom Indexing Kit D | 96 Combinations, 192 Reactions | IDX-PI-1004-192 |