oncoReveal®
Multi-Cancer v4 with CNV Panel
The oncoReveal® Multi-Cancer v4 with CNV Panel is a robust NGS assay that interrogates genes of interest* across multiple solid tumor cancer types. The panel uses proprietary Stem-Loop Inhibition-Mediated amplification (SLIMamp®) technology, a tiled amplicon-based library prep chemistry, designed by Pillar’s AI-empowered VersaTile™ Primer Design tool, for efficient single-tube target enrichment. In addition to SNVs and indels, the assay detects CNVs in 14 genes.
oncoReveal® Multi-Cancer v4 with CNV Panel (60 genes)
Variants and CNVs detected from DNA
| ABL1 | CDKN2A | FBXW7 | GNAS | KIT | NPM1 | PTPN11 | SRC |
| AKTI | CSF1R | FGFR1 | HNF1A | KRAS | NRAS | RAC1 | STK11 |
| ALK | CTNNB1 | FGFR2 | HRAS | MAP2K1 | NTRK1 | RB1 | TP53 |
| APC | DDR2 | FGFR3 | IDH1 | MET ◼ | NTRK2 | RET | VHL |
| ATM | EGFR ◼ | FLT3 | IDH2 | MLH1 | NTRK3 | ROS1 | |
| BRAF | ERBB2 ◼ | FOXL2 | JAK2 | MPL | PDGFRA | SMAD4 | |
| CCNE1 | ERBB4 | GNA11 | JAK3 | MYC ◼ | PIK3CA | SMARCB1 | |
| CDH1 | EZH2 | GNAQ | KDR | NOTCH1 | PTEN | SMO |
| ABL1 | FBXW7 | KIT | PTPN11 |
| AKTI | FGFR1 | KRAS | RAC1 |
| ALK | FGFR2 | MAP2K1 | RB1 |
| APC | FGFR3 | MET ◼ | RET |
| ATM | FLT3 | MLH1 | ROS1 |
| BRAF | FOXL2 | MPL | SMAD4 |
| CCNE1 | GNA11 | MYC ◼ | SMARCB1 |
| CDH1 | GNAQ | NOTCH1 | SMO |
| CDKN2A | GNAS | NPM1 | SRC |
| CSF1R | HNF1A | NRAS | STK11 |
| CTNNB1 | HRAS | NTRK1 | TP53 |
| DDR2 | IDH1 | NTRK2 | VHL |
| EGFR ◼ | IDH2 | NTRK3 | |
| ERBB2 ◼ | JAK2 | PDGFRA | |
| ERBB4 | JAK3 | PIK3CA | |
| EZH2 | KDR | PTEN |
CNVs detected and verified by NIST reference standard are indicated by ◼ CNVs can also be detected in genes indicated by
Simple NGS Library Prep Workflow
Maintain control of samples and results with single-tube, tiled amplification that can be performed in-house by any NGS labSensitive and Robust Chemistry
Achieve variant detection as low as 1% VAF* even with limited DNA input or poor sample qualityReduced Fully-Loaded Lab Costs
Improve lab efficiency and reduce “no calls”, repeat testing, and difficult interpretation decisions*VAF, variant allele frequency
For Research Use Only. Not for use in diagnostic procedures..
Panel Specifications*
| Enrichment chemistry | Multiplex PCR using tiled amplicons |
| Number of pools | 1 pool |
| Number of genes/amplicons | 60/341 |
| Number of targets | Hotspots in 60 genes; CNVs for 14 genes; ~33.1kb total size |
| Variant types | SNVs, small and medium indels, and CNVs |
| Average amplicon size | 143bp |
| Recommended input range | 5ng to 80ng DNA |
| Sample types | DNA from tissue, blood; or FFPE |
| Mapping rate | 99.23% ± 0.3% |
| % on-target aligned reads | 99.5% ± 0.1% |
| Coverage uniformity (% targets with >0.2x mean coverage) | 98.2% ± 0.7% |
| Recommended reads per sample | ~1.4 million paired-end reads |
| Total assay time (from RNA to sequencer) | <8 hours |
*Mapping rate, percentage of on-target aligned reads, and coverage uniformity metrics are based on internal testing performed using reference standard materials
Ordering Information
Select the panel AND one of the index kit options listed below.
| Panel | Part number |
|---|---|
| oncoReveal® Multi-Cancer v4 with CNV Panel (24 reactions) | HDA-HS-1002-24 |
| Pillar Index Kit options | Reactions | Part number |
|---|---|---|
| Pillar Custom Index Primers Kit A | 32 Combinations, 96 reactions | IDX-PI-1001-96 |
| Pillar Custom Index Primers Kit D | 96 Combinations, 192 reactions | IDX-PI-1004-192 |
User Guide
Multi-Cancer v4 with CNV Panel
Product Sheet
Multi-Cancer v4 with CNV Panel
SDS Sheet
Multi-Cancer v4 with CNV Panel