oncoReveal®
Rapid AML Panel
The oncoReveal® Rapid AML Panel is a robust NGS assay that interrogates 9 genes of interest most relevant to acute myeloid leukemia (AML). The panel uses proprietary Stem-Loop Inhibition-Mediated amplification (SLIMamp®) technology, a tiled amplicon-based library prep chemistry, designed by Pillar’s AI-empowered VersaTile™ Primer Design tool, for efficient single-tube target enrichment.
oncoReveal® Rapid AML Panel (9 genes)
| CEBPA | IDH2 | NPMA1 |
| FLT3 (ITD) | KIT | NRAS |
| IDH1 | KRAS | TP53 |
Genes marked in green indicate full CDS coverage
Simple NGS Library Prep Workflow
Maintain control of samples and results with single-tube, tiled amplification that can be performed in-house by any NGS labSensitive and
Robust Chemistry
Achieve variant detection as
low as 2% VAF* even with
limited DNA input or poor
sample quality
Reduced Fully-Loaded
Lab Costs
Improve lab efficiency
and reduce “no calls,”
repeat testing, and difficult
interpretation decisions
*VAF, variant allele frequency
For Research Use Only. Not for use in diagnostic procedures..
Panel Specifications*
| Enrichment chemistry | Multiplex PCR using tiled amplicons |
| Number of pools | 1 pool |
| Number of genes/amplicons | 9/57 |
| Number of targets | Hotspot coverage in 7 genes, full CDS coverage of TP53 and CEBPA, FLT3 ITDs |
| Variant types | SNVs, indels, ITD (internal tandem duplicates) |
| Average amplicon size | 214bp |
| Recommended DNA input range | 20ng to 60ng |
| Sample types | DNA from whole blood, PBMCs, WBCs, or bone marrow |
| Mapping rate | ~99% |
| % on-target aligned reads | ~98% |
| Coverage uniformity (% targets with >0.2X mean coverage) | ~95% |
| Recommended Reads Per Sample | 306,000 paired-end reads |
| Total assay time (from DNA to sequencer) | <8 hours |
*Mapping rate, percentage of on-target aligned reads, and coverage uniformity metrics are based on internal testing performed using reference standard materials
Ordering Information
Select the panel and one of the index kit options listed below.
| Panel | Part number |
|---|---|
| oncoReveal® Rapid AML Panel (24 reactions) | HDA-MY-1007-24 |
| Pillar Index Kit options | Reactions | Part number |
|---|---|---|
| Pillar Custom Index Primers Kit A | 32 Combinations, 96 reactions | IDX-PI-1001-96 |
| Pillar Custom Index Primers Kit D | 96 Combinations, 192 reactions | IDX-PI-1004-192 |