oncoReveal®
Solid Tumor 22 Gene Panel
The oncoReveal® Solid Tumor 22 Gene Panel is a robust NGS assay that interrogates 22 genes of interest* relevant to lung and colon cancer. The panel uses proprietary Stem-Loop Inhibition-Mediated amplification (SLIMamp®) technology, a tiled amplicon-based library prep chemistry, designed by Pillar’s AI-empowered VersaTile™ Primer Design tool, for efficient single-tube target enrichment.
oncoReveal® Solid Tumor 22 Gene Panel
| AKT1 | EGFR | FGFR2 | NOTCH1 | STK11 |
| ALK | ERBB2 | FGFR3 | NRAS | TP53 |
| BRAF | ERBB4 | KRAS | PIK3CA | |
| CTNNB1 | FBXW7 | MAP2K1 | PTEN | |
| DDR2 | FGFR1 | MET | SMAD4 |
Simple NGS Library Prep Workflow
Maintain control of samples and results with single-tube, tiled amplification that can be performed in-house by any NGS labSensitive and Robust Chemistry
Achieve variant detection as low as 1% VAF* even with limited DNA input or poor sample qualityReduced Fully-Loaded Lab Costs
Improve lab efficiency and reduce “no calls”, repeat testing, and difficult interpretation decisionsPanel Specifications*
| Enrichment chemistry | Multiplex PCR using tiled amplicons |
| Number of pools | 1 pool |
| Number of genes/amplicons | 22/103 |
| Number of targets | >1,800 hotspots in 22 genes; 10.3kb total size |
| Variant types | SNVs, indels |
| Average amplicon size | 147bp |
| Recommended DNA input range | 10ng to 80ng |
| Sample types | DNA from tissue, blood, or FFPE |
| Mapping rate | 97.4% ± 2.5% |
| % on-target aligned reads | 96.8% ± 1.3% |
| Coverage uniformity (% targets with >0.2X mean coverage) | 99.8% ± 0.7% |
| Recommended reads per sample | ~475,000 paired-end reads |
| Total assay time (from DNA to sequencer) | <8 hours |
* Mapping rate, percentage of on-target aligned reads, and coverage uniformity metrics are based on internal testing performed using reference standard materials
Ordering Information
Select the panel AND one of the index kit options listed below.
| Panel | Part number |
|---|---|
| oncoReveal® Solid tumor 22 Gene Panel (24 reactions) | HDA-ST-1001-24 |
| Pillar Index Kit options | Reactions | Part number |
|---|---|---|
| Pillar TSCA Index Primers Kit A | 32 Combinations, 96 reactions | IDX-AI-1001-96 |